![]() A blue line shows a fitted sigmoid curve. Center line, median box limits, upper and lower quartiles whiskers, 1.5x interquartile range points, outliers. The plots of the ONM/INM distance and the tip curvature are from 71, 75, 88, 64, 58, and 52 pores at 4.8, 6.1, 7.7, 10, 15 min, and in interphase, respectively. ( b, c) Quantification of the distance between ONM and INM ( b) and the radius of membrane tip curvature ( c) as indicated by a blue bidirectional arrow and a red arrow in the left panels. ONM, outer nuclear membrane INM, inner nuclear membrane. ( a) Other images of pre-pores at different times after AO (4.8, 6.1, 7.7, 10, and 15 min). Generation and validation of homozygous fluorescent knock-in cells using genome editing. Comparative assessment of fluorescent transgene methods for quantitative imaging in human cells. in Proceedings of the 21st International Conference on Machine Learning 30 (2004). Linearized cluster assignment via spectral ordering. A spectral algorithm for seriation and the consecutive ones problem. A multilinear singular value decomposition. Nuclear pore complex structure and dynamics revealed by cryoelectron tomography. Microscopy image browser: a platform for segmentation and analysis of multidimensional datasets. of the IEEE International Symposium on Biomedical Imaging 230‒233 (IEEE, Piscataway, New Jersey, 2011).īelevich, I., Joensuu, M., Kumar, D., Vihinen, H. ilastik: Interactive Learning and Segmentation Toolkit. ![]() Computer visualization of three-dimensional image data using IMOD. Variational algorithms to remove stationary noise: applications to microscopy imaging. TrakEM2 software for neural circuit reconstruction. Multi-resolution correlative focused ion beam scanning electron microscopy: applications to cell biology. SiR-Hoechst is a far-red DNA stain for live-cell nanoscopy. Cellular reorganization during mitotic entry. Interaction of Nup53 with Ndc1 and Nup155 is required for nuclear pore complex assembly. Dimerization and direct membrane interaction of Nup53 contribute to nuclear pore complex assembly. Molecular architecture of the inner ring scaffold of the human nuclear pore complex. Steps of nuclear pore complex disassembly and reassembly during mitosis in early Drosophila embryos. Dimples, pores, star-rings, and thin rings on growing nuclear envelopes: evidence for structural intermediates in nuclear pore complex assembly. Progressive sheet-to-tubule transformation is a general mechanism for endoplasmic reticulum partitioning in dividing mammalian cells. Puhka, M., Joensuu, M., Vihinen, H., Belevich, I. Importin beta regulates the seeding of chromatin with initiation sites for nuclear pore assembly. The conserved Nup107-160 complex is critical for nuclear pore complex assembly. Nuclear pore assembly proceeds by an inside-out extrusion of the nuclear envelope. Formation of the postmitotic nuclear envelope from extended ER cisternae precedes nuclear pore assembly. Inner/outer nuclear membrane fusion in nuclear pore assembly: biochemical demonstration and molecular analysis. Nuclear reformation at the end of mitosis. Enclosing chromatin: reassembly of the nucleus after open mitosis. Nuclear pore complex assembly through the cell cycle: regulation and membrane organization. Dynamics of nuclear pore complex organization through the cell cycle. Imaging the assembly, structure, and function of the nuclear pore inside cells. ![]() Systematic kinetic analysis of mitotic dis- and reassembly of the nuclear pore in living cells. Perforating the nuclear boundary - how nuclear pore complexes assemble. Mechanisms and functions of nuclear envelope remodelling. The nuclear pore complex: understanding its function through structural insight. ![]()
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